BIKES will be allowed on the DLR following a successful trial.
Cyclists will be able to take their non-folding bicycles on the trains during off-peak times.
More than 5,000 cyclists travelled with their bikes on the DLR during a successful trial period that started last July.
Mayor of London Boris Johnson said: "Opening up the Docklands Light Railway to cyclists will be a great boost to the cycling community and make it much more convenient for cyclists to cross the river.
"This is another important step forward in our mission to make it easier for more people to get cycling in the capital."
Folding bicycles are permitted on the DLR at all times, including during peak hours.
From January 27 2014, non-folding bicycles will be allowed on DLR trains from Monday to Friday during off-peak hours and all day at weekends and bank holidays.
For safety reasons, bicycles are not allowed on trains travelling between Shadwell and Bank stations.